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Test-Category
ELISA
Pack size
96 Tests
Standard concentrations included in the kit
370-30000 pg/ml
Possible sample matrix and volumes
| Serum | 50 µl |
| Plasma | 50 µl |
Incubation periods
Indications
Regulatory Status
RANKL, receptor activator of nuclear factor (NF)-kB ligand (also: osteoprotegerin ligand, OPGL), its cellular receptor, receptor activator of NF-kB (RANK), and the decoy receptor, osteoprotegerin (OPG) have been identified as the key molecular regulation system for bone remodelling. RANKL, a member of the tumor necrosis factor (TNF) family, is the main stimulatory factor for the formation of mature osteoclasts and is essential for their survival. Therefore, an increase in RANKL expression leads to bone resorption and bone loss. RANKL is produced by osteoblastic lineage cells and activated T lymphocytes. It activates its specific receptor RANK which is located on osteoclasts and dendritic cells.
The effects of RANKL are counteracted by OPG which is secreted by various tissues and acts as an endogenous soluble receptor antagonist.
The concentration of free sRANKL is rather low in human serum or plasma. That is why the determination of the very same is difficult in those matrices. This fact which lies in the nature of the parameter if measured in serum or plasma, inspired Immundiagnostik to develop a new kit for measuring total sRANKL.
The antibody combination in our total sRANKL ELISA detects the OPG-sRANKL-complex. Running the assay with an excessive addition of OPG to the sample, the primarily present OPG-sRANKL-complex plus the newly evoked OPG-sRANKL-complex are detected. Running the assay without an ecxessive addition of OPG to the sample, only the primarily present OPG-sRANKL-complex in the sample is detected. Subtracting Value 2 from Value 1 will give you the value of free sRANKL in the sample.
